Skip to content
Dymatize ISO100 Hydrolyzed Whey Protein Isolate
Best Whey IsolateProtein Powder

DymatizeISO100 Hydrolyzed Whey Protein Isolate

Hydrolyzed whey isolate: 25 g protein, 1 g sugar or less and cult-favorite flavors

By FitnessReviews Editors6 sourcesMore from Dymatize

#2Ranked #2 in The Best Protein Powders of 2026
FR Score 8.9 out of 10, Excellent

FR Score

Excellent

Built from 5 independent video reviews, label & spec analysis, and value. How we score

An editorial research score. We have not verified hands-on testing by our editors for this product.

Check price on AmazonShop on TikTokBuy from the brand

We may earn a commission when you buy through our links — it never affects our scores. How we make money

Protein per serving
25 g
Calories per serving
120 or less
Sugar per serving
1 g or less
BCAAs per serving
5.5 g (2.6 g leucine)
Watch 5 reviews

The verdict

ISO100 is the go-to isolate for lean macros and easy digestion, with 25 g of protein, 120 calories or less and 1 g of sugar or less per scoop, all from whey isolate. Reviewers rave about flavors like Fruity Pebbles and Gourmet Chocolate. The trade-off is a higher price per serving than whey blends.

Check price on Amazon

Affiliate link — we may earn a commission. It never affects our scores.

Score breakdown

8.9 / 10
Formula & Dosing9.2
Quality & Transparency9.0
Taste & Mixability9.2
Value per Serving8.2

What we like

  • 100% of protein from whey isolate, partly hydrolyzed
  • 1 g of sugar or less and 120 calories or less per 25 g protein scoop
  • Standout flavors, including Pebbles cereal collaborations
  • Banned-substance tested; retailers list Informed Choice certification
  • Very fine powder that mixes quickly in water

Watch out for

  • Pricier per serving than whey blends
  • Flavored versions use sucralose and soy lecithin
  • Some flavors taste very sweet

Full review

Overview

Dymatize ISO100 uses hydrolyzed whey protein isolate: whey filtered to strip out most lactose, fat and carbs, then partially broken into smaller peptides for fast digestion. Per scoop you get 25 g of protein, 5.5 g of BCAAs including 2.6 g of leucine, 120 calories or less and 1 g of sugar or less. Dymatize labels it banned-substance tested, and major retailers list it as Informed Choice certified. Flavored versions are sweetened with sucralose and contain soy lecithin.

What reviewers say

The consensus from dietitians, coaches and taste-testers is that this is one of the best-tasting isolates you can buy. Fruity Pebbles, Cocoa Pebbles and Gourmet Chocolate earn repeat praise, although a few TikTok reviewers find Fruity Pebbles very sweet. The powder is extremely fine and mixes into a thin, slightly frothy shake in water. Garage Gym Reviews calls it delicious and recommends it to anyone prioritizing protein with minimal carbs or fat, while coach Dr. Marc Morris devotes part of his review to who should skip it, since you are paying extra for the hydrolyzed isolate.

Who it's for

Lifters in a cutting phase who want maximum protein per calorie, people who feel bloated on concentrate-heavy blends, and tested athletes who want a certified product with a deep flavor menu. It also suits anyone who prefers a light, juice-thin shake after training rather than a thick, milkshake-style drink.

Bottom line

If you want an isolate that genuinely tastes good, ISO100 is the safe bet. At about $1.55 per serving for the 5 lb tub, it costs more than blends, but you get cleaner macros and very low lactose for the money.

Specs & details

Protein per serving
25 g
Calories per serving
120 or less
Sugar per serving
1 g or less
BCAAs per serving
5.5 g (2.6 g leucine)
Protein source
Hydrolyzed whey protein isolate and whey protein isolate
Servings
71 per 5 lb tub (Gourmet Chocolate)
Sweetener
Sucralose
Allergens
Milk, soy (soy lecithin)
Third-party testing
Banned-substance tested (Informed Choice per retailer listings)

Frequently asked questions

What does hydrolyzed mean on ISO100?
Hydrolyzed protein has been partially broken down into smaller peptides during processing. Dymatize uses it to speed digestion and absorption. In practice, most people notice the benefits of isolate itself, which include very little lactose, fat or sugar, more than the hydrolysis, which is one reason ISO100 costs more than standard whey blends.
Is ISO100 a good choice if dairy upsets my stomach?
ISO100 is filtered to remove most lactose, and many lactose-sensitive users say they tolerate it better than concentrate-based wheys. It is still a milk product and contains soy lecithin, so it is not dairy-free. If you are highly sensitive, start with a half scoop or choose a plant-based protein instead.
Is Dymatize ISO100 third-party tested?
Dymatize labels ISO100 as banned-substance tested, and major retailers such as iHerb describe it as Informed Choice certified. Consumer Reports did not include ISO100 in its October 2025 heavy-metal testing; the Dymatize product CR tested was its Super Mass Gainer, so there is no CR lead result for ISO100 itself.

This review is for information only and isn’t medical advice. Supplements aren’t reviewed by the FDA before sale — check with a doctor or pharmacist before starting one, especially if you’re pregnant, take medication or have a health condition. Health disclaimer

Compare

ISO100 Hydrolyzed Whey Protein Isolate vs. the alternatives

See all 5 ranked
Dymatize ISO100 Hydrolyzed Whey Protein Isolate
DymatizeISO100 Hydrolyzed Whey Protein Isolate
Optimum Nutrition Gold Standard 100% Whey
Optimum NutritionGold Standard 100% Whey
Transparent Labs Grass-Fed Whey Protein Isolate
Transparent LabsGrass-Fed Whey Protein Isolate
Nutricost Whey Protein Concentrate
NutricostWhey Protein Concentrate
FR Score8.9 out of 10FR ScoreExcellent9.1 out of 10FR ScoreExceptional8.8 out of 10FR ScoreExcellent8.2 out of 10FR ScoreGreat
Best forBest Whey IsolateBest OverallBest Clean LabelBest Budget
Typical priceSee retailer~$131.99~$64.99See retailer
Video reviews5544
Where to buyAmazon · TikTok Shop · BrandAmazon · TikTok Shop · BrandAmazon · TikTok Shop · BrandAmazon · TikTok Shop · Brand

From the blog

Read more about the ISO100 Hydrolyzed Whey Protein Isolate

All articles

Keep exploring

More in Protein Powder

View all
  • Optimum Nutrition Gold Standard 100% Whey
    Best Overall
    FR Score 9.1 out of 10, Exceptional
    Optimum NutritionProtein Powder

    Gold Standard 100% Whey

    The benchmark whey: 24 g protein, Informed Choice certified, 15+ flavors

    5 videos
    About ~$131.99
    AmazonTikTok ShopBrand store
  • Transparent Labs Grass-Fed Whey Protein Isolate
    Best Clean Label
    FR Score 8.8 out of 10, Excellent
    Transparent LabsProtein Powder

    Grass-Fed Whey Protein Isolate

    28 g grass-fed isolate, stevia-sweetened, certified and batch-tested

    4 videos
    About ~$64.99
    AmazonTikTok ShopBrand store
  • Nutricost Whey Protein Concentrate
    Best Budget
    FR Score 8.2 out of 10, Great
    NutricostProtein Powder

    Whey Protein Concentrate

    No-frills 25 g whey concentrate that reviewers name their budget pick

    4 videos
    AmazonTikTok ShopBrand store
  • Orgain Organic Protein Plant-Based Protein Powder
    Best Vegan
    FR Score 7.9 out of 10, Good
    OrgainProtein Powder

    Organic Protein Plant-Based Protein Powder

    USDA Organic pea, rice and chia blend: 21 g vegan protein, 7 g fiber, no added sugar

    4 videos
    About ~$37.99
    AmazonTikTok ShopBrand store
  • Sports Research Triple Strength Omega-3 Fish Oil
    Best Overall
    FR Score 9.3 out of 10, Exceptional
    Sports ResearchVitamins & Wellness

    Triple Strength Omega-3 Fish Oil

    950 mg of EPA + DHA in one softgel, IFOS 5-Star rated, for about 33 cents a day.

    4 videos
    About ~$29.79
    AmazonTikTok ShopBrand store
  • Optimum Nutrition Micronized Creatine Powder
    Best Overall
    FR Score 9.2 out of 10, Exceptional
    Optimum NutritionCreatine

    Micronized Creatine Powder

    5 g micronized monohydrate per scoop, Informed Choice tested, about 23 cents a serving

    4 videos
    AmazonTikTok ShopBrand store
  • CELSIUS Sparkling Energy Drink
    Best Overall
    FR Score 9.1 out of 10, Exceptional
    CELSIUSEnergy Drinks

    Sparkling Energy Drink

    The original fitness energy drink: 200 mg caffeine, 10 calories and zero sugar per can.

    4 videos
    AmazonBrand store
  • Transparent Labs BULK Pre-Workout
    Best Overall
    FR Score 9.0 out of 10, Exceptional
    Transparent LabsPre-Workout

    BULK Pre-Workout

    Clinically dosed, stevia-sweetened and Informed Choice certified

    5 videos
    About ~$49.99
    AmazonTikTok ShopBrand store